The underlying data requirement is in place to ensure that the results are reported in a fully transparent manner, and that readers can verify results by reviewing the primary data in its original form. The following requirements apply to any figures and supporting Information files that report blot or gel data. 100dpi works well for medium sized email. 0 Comments All, very easy solution to reduce file size of pdf as follows: In Acrobat Pro, select save as Then under format select Adobe PDF files, optimized Click settings Then adjust your image downsampling as you like. How Avoid Reducing Image Size In Word For Mac Convert Pdf.Click OK to reduce the resolution. Select the compression you would like for the picture (the smaller the DPI, the smaller the size of the image). Document comparison, PDF creation, and image file conversion to PDF.Under Picture Tools, on the Format tab, in the Adjust group, click Compress Pictures. If you have questions about these requirements please email us at Original images for blots and gelsWith the fastest Microsoft Office to PDF converter on the market, our Batch tool. The figure preparation guidelines that follow clarify PLOS ONE standards and requirements, and aim to ensure the integrity and scientific validity of blot/gel data reporting.Any non-specific bands from the original image should be included in the figure and explained in the text or figure legend. Figure panels should include some background area above and below bands. Images in figures should not be overcropped around the bands of interest. Any adjustments to improve visibility of the data must be consistently applied to the entire image. The background and contrast should not be modified to notably alter the visibility of data, background, or non-specific bands.
Please also see our guidelines on Preparing Figures from Image Files for further details.The following guidelines aim to help authors prepare high quality figures and avoid common errors that result from inappropriate image manipulation. This would be appropriate, for example, if fragments of the same original image were spliced together to re-order lanes or to remove irrelevant lanes. If lanes from a single blot/gel image have been rearranged in preparing a figure, image splicing should be clearly denoted by vertical black lines on the figure and the figure legend should provide details of how the figure was made. If data from multiple blot or gel images are needed to illustrate the results, the different images should not be spliced together they should be clearly distinguished as separate panels within the figure and the legend should note that they represent different gels, blots, or exposures. A figure panel should not include composite images of bands originating from different blots, exposures, or gels. Each figure should include all relevant controls, and when relevant, control samples should be run on the same blot or gel as the experimental samples. EPS files often have missing/corrupted fonts, oversized masks, stray points, and boxes, which can result in errors and poor output.At the height maximum, the figure occupies the whole page and excludes the caption“Dimensions” refers to the dimensions of the entire figure, excluding any white space. If evidence is found of inappropriate manipulation, we reserve the right to ask for original data and, if that is not satisfactory, we may decide not to accept the manuscript, and may also contact the authors' institutions to ask them to assist with investigation.Should I choose TIFF or EPS? TIFF tends to be easier to work with than EPS. Please refer to the general guidance below on aspects to consider when preparing your figures. Avoiding inappropriate image manipulationWe realize that the extent to which figures can be changed as part of normal preparation can pose a dilemma. Additionally, if further analysis is needed, please be aware that we may also require you to supply us with your original photographic images in the original file format and at the resolution at which they were first created. Acounting software for macThe quality of a low-resolution figure cannot be improved by simply increasing the resolution in graphics software. Go to Image → Properties (or Image → Information) to view image dimensions.Submit figures at the desired dimensions with a resolution no greater than 300-600 dpi. This will maintain the width and height ratio of your figure. When adjusting the figure dimensions, be sure the Chain/Lock symbol is closed. To align your figure with the text column of the PDF version of the article, make it no wider than 5.2 inches (13.2 cm).Ensure that individual images meet the pixel dimensions indicated above when preparing and saving figures with slideshow software (like Powerpoint, OpenOffice, or Keynote).Make sure source images are 2,250 pixels wide if you want figures at full page width of 19.05cm. A flattened TIFF has a single layer called “background” and has a smaller file size than a TIFF with “Layer 1”. If you elect not to use PACE, follow the instructions below to manually resize TIFF or EPS files. Use the PACE tool for help resizing. If you created a 72 dpi line graph and placed it in a 300 dpi TIFF, the graph will look blurred, jagged, or pixilated.Submit files at a size of 10 MB or less. Resolution below 300 results in blurred, jagged or pixelated published figures. Resolution above 600 dpi may lead to resizing of the published figure.The quality of your figures is only as good as the lowest-resolution element present. How Avoide Reducing Image Size In Word Convert Update All FigureFormat your figure captions. Place figure captions in the manuscript text in read order, immediately following the paragraph where the figure is first cited. Do not include captions as part of the figure files or submit them in a separate document. Read more about submitting captions.If at any point you change the numbering order of your figures, be sure to update all figure citations, captions, and file names accordingly. That information will go into your figure caption in the manuscript. If your EPS files are still too large, convert them to PDF and then export to a compressed TIFF.Use only Arial, Times, or Symbol font in 8-12 point.Do not include author names, article title, or figure number/title/caption within figure files. Restrict it to 15 words or less.Legend. Place the legend directly after the title of the figure to which it belongs. Legends are optional.Label. Name figure labels using Arabic numerals, and abbreviate the word “Figure” to “Fig” (e.g., Fig 1, Fig 2, Fig 3, etc.).Title. The title should be concise and descriptive.
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